Discovering DNA Packaging into the Nucleosome

Discovering DNA Packaging

Dna Packaging is the folding of DNA to fiit a cell’s limited space. This process differs greatly from Prokaryotic cells to Eukaryotic cells and is much better understood in Eukaryotes. The packaging unit of DNA is called the nucleosome which is basically 8 proteins with 2 DNA strands wrapped around it 2 times. These histone proteins include: H1, H2A, H2B, H3, and H4. When Chromatin (DNA that hasn’t condensed into chromosomes) is put into a low salt solution, it takes the form of “Beads on a string”. It literally looks like those Mardi gras necklaces. When Chromatin is put into a solution with 0.15M KCL, takes the form of the “30-nm fiber” where the DNA now looks like a long thick snake.

DNA packaging has a big problem:

1. The longest human chromosome has 3 X 10^8 base pairs (adenine, cytosine, guanine, thymine). That is equivalent to 10cm. The problem is fitting that much DNA into a cell. A typical cell is 10 micrometers and therefore DNA needs to be compacted 10,000X.

How was the nucleosome (packaging unit of DNA) discovered?

The Experiment:

1. The Nuclei was isolated

2. DNAse I (an enzyme which cuts DNA at random locations by breaking phosphodiester bonds) was used to partially digest the DNA (Chop the DNA into fragments).

3. Run the DNA fragments through an agarose gel (which separates the DNA by size)

Result: Scientists found that the DNA fragments were 150- 200 base pairs long (This is weird because DNAse I cuts the DNA at RANDOM places and DNA is VERY long. Because each fragment were about the same length as the next one it means something is blocking the DNA from being randomly chopped up.

Conclusion: This means something is protecting the DNA and that is the histones: H1, H2A, H2B, H3, and H4

To be more precise, the scientists did this:

4. Put in more DNAse so the DNA could be cut up further

5. Run the DNA through Acrylamide gel instead of agarose which provides a better separation

Result: They found that the DNA Fragments are now multiples of 10 – 11 base pairs long. VERY precise.

Conclusion: DNA must be wrapped around the outside of the nucleosome. This also shows that the DNA is bDNA because it is always around 10 base pairs.

More by this Author


Comments 1 comment

TFScientist profile image

TFScientist 4 years ago from Peterborough, UK

Excellent description of the nucleosome experiment. There are a number of visuals available for apoptosis that show CAD cutting between the nucleosomes during fragmentation of chromatin in...you guessed it...ladders of multiples of 11. Nice Hub.

    Sign in or sign up and post using a HubPages Network account.

    0 of 8192 characters used
    Post Comment

    No HTML is allowed in comments, but URLs will be hyperlinked. Comments are not for promoting your articles or other sites.


    Click to Rate This Article
    working