Ion exchange chromatography: Principle, method & applications
Ion exchange chromatography
Ion exchange chromatography is a process of separation of molecules of like or same charges from a mixture by use of ion exchange resins.
Principle: The separation occurs by reversible exchange of ions between the ions present in the solution and those present in the ion exchange resin.
Ion exchange resins like anion exchange for anions and cation exchange resins for cation separation are employed.
The ion retained in the resin matrix are removed by use of similar strong charged mobile phase.
Resin-H+ +M+ (in solution) ------------> Resin-M+ + H+ (in solution).
Resin-OH- +A- (in solution) ------------> Resin-A- + OH-(in solution).
Many drugs in pharmaceutical products are either acidic or basic in nature. Their mixture can be separated into pure compound by Ion exchange chromatography.
Applications of ion exchange chromatography.
Ion exchange chromatography is prominently used as preparatory chromatography to isolate a desired compound from mixture. Hence the applications are meant to obtain pure compounds.
a) For deionisation and softening of water.
b) Purification of solution to keep them ion free.
c) In biochemistry for separation of drugs and metabolites from blood, urine etc.
d) Separation of organic mixture of acidic and basic compounds.
e) For extraction of enzymes from tissues.
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