A Simple Introduction to Tissue Identification

Through the Microscope

I have put together this guide for the identification of tissue types and where they are found within the body to help students of Histology.

The following is an attempt at organizing information necessary to not only pass a graduate level Histology course but to aid with the studying for the United States Medical Licensing Examination (USMLE).

This information is provided from my personal experience in Histology and a beleif that I can organize the information in a way that will be useful for those who are about to study for the above mentioned exams.

For those of you who have no experience with Histology and are not currently working on a Medical License or the lay person who wants to learn more about Anatomy and Physiology, take your time, and use the comment section to ask as many questions you may have.

Basic Cell Structure

The cell is a structurally independent unit and may function independently or as a group. There are two major parts of the cell, the nucleus and the cytoplasm.

The cells have organelles that are responsible for the specialized functions of the cell. for example the ribosomes for protein synthesis, or vacoules for storage, or mitochondria for respiration.

All living organisms are composed of cells as their basic functional units.

Cytology is the study of cells, in this article we are more concerned with Histology or the study of tissue.


Tissue Layers and Organs

Our body is made of organ systems, organs, tissues, and cells. Sometimes to understand the physiology of an organ system, or an organ within a system, it is necessary to look at the cells that make up the tissue within the organ.

I will be going over the different epithelial tissue, connective tissue (including bone and blood cells), muscle tissue, and nervous tissue, and where these tissues are located in the body.

A discussion of function may not be included with the tissue types or an indepth look at physiology. There is far too much information to fit here, but it is important to remember that form and function lie hand in hand.

Simple Squamous Epithelium H&E Stain
Simple Squamous Epithelium H&E Stain
Simple Cuboidal H&E Stain
Simple Cuboidal H&E Stain
Simple Columnar H&E Stain
Simple Columnar H&E Stain
Pseudostratified Columnar H&E Stain
Pseudostratified Columnar H&E Stain
Stratified Squamous H&E Stain
Stratified Squamous H&E Stain
Stratified Cuboidal H&E Stain
Stratified Cuboidal H&E Stain
Stratified Columnar H&E Stain
Stratified Columnar H&E Stain
Transitional Epithelium H&E Stain
Transitional Epithelium H&E Stain

Epithelial Tissue

Epithelium covers all external surfaces of the body and lines the internal surfaces of organs. The free surface of epithelium has contact with either air or fluid.

The major function of Epithelium is to cover and line body surfaces. Modifications on the surface of epithelial cells is dependent on their functions. They can be either in single layers or multiple layers and always rest upon a basement membrane.

Simple squamous Epithelium is a single layer of flat or elongated cells. It is located in the lining of the Heart, the Alveoli of the Lungs, the Glomerulus of the Kidney, the inner surfaces of the Tympanic Membrane, the layer of Peritonium, and Lymph Vessels.

Simple squamous Endothelium is a single layer of flat or elongated cells having a lumen which contains blood. It is located in Arteries and Veins.

Simple Cuboidal Epithelium is a single layer of round to square shaped cells attached to a basement membrane. It is located on the surface of an ovary, the pigments epithelium at the posterior surface of the eye, the kidney tubules, the ducts of many glands, the secretory portions of some endocrine glands including the thyroid and pancreas.

Simple Columnar Epithelium is a single layer of cells that are taller than they are wide. They may be ciliated or nonciliated. Ciliated Simple Columnar Epithelium is located in the upper Respiratory Tract, the Fallopian Tubes, Brain Ventricles, and the Central Canal of Spinal Cord. Nonciliated Simple Columnar Epithelium is located on the surface of gastrintestinal tract, the ducts of many glands, and the Gallbladder.

Psuedostratified Columnar Epithelium is actually a single layer of cells, taller than they are wide, which appears to be arranged in multiple layers. Individual cells can be seen extending from the basement membrane to the lumen. They are found in the Epidiymis and parts of the male Urethra.

Ciliated Pseudostratified Columnar Epithelium is same as mentioned above yet has cilia on the outer border facing the lumen. These are found in the upper respiratory tract.

Stratified Squamous Epithelium is multiple layers of cells flattened at the luminal surface. They may be keratonized or nonkeratonized. The keratonized is located in thick and thin skin. The nonkeratonized is located on the lining of the mouth, on the larynx, on the epiglottis, on the Vagina, and on the Tongue.

Stratified Cuboidal Epithelium is two or three layers of round to square cells on the luminal surface. These are located in adult sweat glands, in Esophageal glands, and in parts of the male urethra.

Stratified Columnar Epithelium is two or more layers of cells elongated on the luminal surface. They line parts of the U rethra, the excretory ducts of some glands, Anal mucous membranes, and part of the conjuctiva of the eye.

Transitional Epithelium is multiple layers of cells whose luminal surface is characterized by loose rounded to cuboidal appearing cells. They are located on the lining of the Urinary Bladder, Ureters, and parts of the Urethra.

Embryonic Connective Tissue Mesenchymal H&E Stain
Embryonic Connective Tissue Mesenchymal H&E Stain
Loose Aerolar Connective Tissue H&E Stain
Loose Aerolar Connective Tissue H&E Stain
Adipose Connective Tissue H&E Stain
Adipose Connective Tissue H&E Stain
Reticular Connective Tissue H&E Stain with Silver Impregnation
Reticular Connective Tissue H&E Stain with Silver Impregnation
Dense Regular Connective Tissue H&E Stain
Dense Regular Connective Tissue H&E Stain
Dense Irregular Connective Tissue H&E Stain
Dense Irregular Connective Tissue H&E Stain
Elastic Connective Tissue H&E Stain
Elastic Connective Tissue H&E Stain

Connective Tissue

Connective tissue are tissues containing cells which connect one type of tissue to another or one body area to another. Some have characteristic fibers or cells, and some have both. It provides support to the overlying Epithelium, provides form for the body, provides storage centers, and provides defense mechanisms.

Embryonic Connective Tissue Mesenchymal Tissue has many irregular shaped Mesenchymal cells in a ground substance containing Reticular Fibers. These are found in skin and developing bone of embryos. It can also be found in adult connective tissue along blood vessels.

Embryonic Connective Tissue Mucoid Tissue is scattered Fibroblasts dispersed within a viscous jellylike ground substance containing Collagen fibers. These are located in the Umbilical Cord of the Fetus.

Loose Areolar Connective Tissue can be identified by irregularly arranged fibers of Collagen, Elastic, and Reticular. There is also the presence of characteristic cells such as Fibroblasts, Macrophages, Plasma cells, Adipocytes, and Mast cells in a semifluid matrix. It is located in the Subcutaneous Layers of the Skin, the deep to the basement layers of the Epidermis, the Lamina Propria of mucous membranes, and around the outer portion of Arteries, Veins, Nerves, and most body organs.

Adipose tissue is identified as a collection of adipocytes with the cytoplasm and nucleus located peripherally. Each Adipocyte has a large central droplet of fat. Adipose It is located in the Subcutanous Layer of Skin, around the Heart, Kidneys, Adrenal Glands, Pancreas, and Intestinal Tracts, Posterior to the Eyeball in the Orbit, in Yellow Bone Marrow, and between Skeletal Muscle Fiber Bundles.

Reticular Connective Tissue or Fibers is identified as a thin network of interlacing fibers and cells made up Glycoprotein coated fine Collagen Fibers. It is located in Liver, Spleen, Lymph Nodes, Red Bone Marrow, and associated with basement membranes around Blood Vessels and Muscles.

Dense Regular Connective Tissue or White Fibrous Connective Tissue is the strongest material in our bodies. It is identified as bundles of Collagen fibers in rows with Fibroblasts present between the rows. It is found in Ligaments, Tendons, and Aponeuroses.

Dense Irregular Connective Tissue is randomly arranged Collagen Fibers with periodic Fibroblasts. It provides flexibility and strength and is located in Fascia beneath Skin, around Muscles and Organs, the Dermis of Skin, in capsules around many organs, in Pericardium of Heart, the Peristeum of Bone, and the Perichondrium of Cartilage, and in Joint Capsules.

Elastic Connective Tissues are freely distributed Elastic Fibers and Fibroblasts. It provides maximal elasticity and is located in walls of Arteries, Lungs, Trachea, Bronchi, Vocal Chords, and Ligaments between Vertebrae.


Hyaline Cartilage H&E Stain
Hyaline Cartilage H&E Stain
Compact Bone Silver Impregnation
Compact Bone Silver Impregnation

Cartilage and Bone

Hyaline Cartilage is identified as numerous Chondrocytes located at the ends of long bones, anterior ends of Ribs, parts of Larynx, Trachea, and Bronchi, and the Fetal Skeleton.

Fibrocartilage is identified as Chondrocytes surrounded by dense bundles of Collagen fibers within the extracellular matrix. It is located in the Pubic Symphosis, Intervertebral Discs, Mensci, and portions of Tendon attached to bone.

Elastic Cartilage is identified as Chondrocytes located in a matrix containing a threadlike network of Elastic fibers. It is located in the Epiglottis, the Ear, and the Eustachian Tubes.

Compact bone is identified as a collection of Osteocytes within Lacunae, arranged in concentric Lamellae. it is very dense material and is used a a storage site for Calcium. It is found in the dense portions of bones.

Spongy Bone or Bone Marrow is Hemopoeitic tissue or the blood forming portion of bone. It is made up of Trabeculae, Megakaryocytes, and Adipocytes. It is located in the deep central portions of bones particularly long bones or the distal and proximal Epiphyses and Metaphyses.

RBC's and WBC's Giemsa Stain
RBC's and WBC's Giemsa Stain

Blood Tissue

Blood tissue is made up of Erythrocytes or red blood cells and Leukocytes or white blood cells. White blood cells are either granular like Neutrophils, Basophils, and Eosinophils or nongranular like Lymphocytes, T-cells and B-cells, and Monocytes. Blood tissue is an integral part of the circulatory system which transports blood cells, contains clotting components, and transports nutrients and gases.

Some other constituents of blood are the platelets or Thrombocytes, and plasma proteins. Blood tissue is located inside Arteries and Veins, Red Bone Marrow, and highly vascular organs such as the Liver, Spleen, Lungs, and Kidneys.

Lymph is an aggregation of lymphocytes, T-cells and B-cells, and are located in Lymph nodes, Thymus, Tonsils, and Peyer's Patches in the Intestinal Tract.

Smooth Muscle H&E Stain
Smooth Muscle H&E Stain
Striated Muscle H&E Stain
Striated Muscle H&E Stain

Muscle Tissue

There is three major types of muscle tissue: Smooth Muscle, Skeletal Muscle, and Cardiac Muscle. Muscle tissue helps with body movement, and with movement of bodily secretions.

Smooth Muscle is located in the skin, for example the Arrector Pili muscle in Dermis, the deep layers of the Gastrointestinal Tract, the deep layers of Arteries and Veins, the Uterus, the Gallbladder, the Urinary Bladder, and the Iris of the Eyes. It can be identified by it's central elongated nucleus and it elongated fusiform cellular shape.

Skeletal Muscle is the Muscles attached to bones by tendons and attached to other muscles. It can be identified by an eccentric nuclei, the presence of striations, and an elongated cellular shape.

Cardiac Muscle is located exclusively in the Heart. This muscle can be identified by a central ovoid nucleus, striations, intercalated discs, and irregular cellular branching patterns.

Nervous Tissue

Nervous tissue is ectodermal in nature, which describes where the tissue would begin differentiation in the embryo. Nervous tissue detects, analyzes, and transmits information received by sensory stimuli. It then coordinates motor, visceral, endocrine, and mental activities.

An area made up of neurons and glial cells is called the matrix.

The conductive cells in the matrix are called neurons and can be unipolar, bipolar, and multipolar.

The supportive cells in the matrix are called Glial Cells. The Glial Cells are Astrocyes, Microglia, Oligodendroglia, and Ependymal cells.

The nervous tissue located in the Brain and Spinal Cord can be Grey Matter or White Matter. Grey matter contains primarily neurons and their dendrites. Aggregations of neurons in the brain are called nuclei. Aggregations of neurons outside the Central Nervous System are called ganglia.

White Matter consists of myelinated and unmyelinated axons travelling from one area to another.

More by this Author


Comments 26 comments

ThoughtSandwiches profile image

ThoughtSandwiches 4 years ago from Reno, Nevada

Jamie,

So you were correct my friend...this is a very detailed look at the information needed to pass those exams. From what's here I would have to surmise that they are very difficult exams! I'm glad you got it done!

Thomas


jhamann profile image

jhamann 4 years ago from Reno NV Author

Thomas-

This is one of those projects that I really only did for my own personal satisfaction. Thank you for taking the time to read it and leaving a comment, I am know officially a year Hubber.

Jamie


ThoughtSandwiches profile image

ThoughtSandwiches 4 years ago from Reno, Nevada

Jamie,

Woot! Congrats on your 1-year mark!

Thomas


Jackie Lynnley profile image

Jackie Lynnley 4 years ago from The Beautiful South

What an informative and well done hub! So much work.

Congrats. ^


ausmedus profile image

ausmedus 4 years ago from Kingstown, St. Vincent and the Grenadines

A very very awesome detail about the tissues.................really worth reading, worth noticing, worth liking , worth commenting.


jhamann profile image

jhamann 4 years ago from Reno NV Author

Thank you Thomas, Jackie, and Sally I appreciate your comments and hope you all have a great day! Jamie


unknown spy profile image

unknown spy 4 years ago from Neverland - where children never grow up.

you make this topic very easy to understand and pleasurable to read.


jhamann profile image

jhamann 4 years ago from Reno NV Author

Thank you unknown spy for taking the time to read this fact filled hub and leaving a comment. I hope all is well. Jamie


unknown spy profile image

unknown spy 4 years ago from Neverland - where children never grow up.

wow and this is what we call learning thru reading.


jhamann profile image

jhamann 4 years ago from Reno NV Author

unknown spy-I am speechless that you have stopped by here twice to learn more about a topic that I find interesting. Thank you. Jamie


cam8510 profile image

cam8510 3 years ago from Columbus, Georgia until the end of November 2016.

Great job here. I enjoyed your descriptions and the helpful photos. I am a traveling histology technician. Headed for Philadelphia in a few days for a contract assignment.


jhamann profile image

jhamann 3 years ago from Reno NV Author

I am excited to meet a fellow Histotech here on Hubpages. I am glad that you found this and enjoyed it, I wrote a few hubs as presentations for my CEU's. Keeping that Licensure up! Great to meet you. Jamie


AUPADHYAY profile image

AUPADHYAY 2 years ago from INDIA, UTTAR PRADESH STATE, KANPUR CITY

Thanks jhamann, for sharing the most valuable info hub.


jhamann profile image

jhamann 2 years ago from Reno NV Author

You are welcome, thank you for reading my hub and being interested in the information I am presenting here. I hope all is well. Jamie


DrGaneshPaudel profile image

DrGaneshPaudel 2 years ago from Lanzhou, China

This is really cool.. You threw me into my first year medical school... Highly informative for those who are interested to know what they are made of.. Thumbs up.


jhamann profile image

jhamann 2 years ago from Reno NV Author

Thank you DrGaneshPaudel for your kind words...I have been pondering a History Of Pathology series. If this ever comes to light I hope to see you have stopped by. Jamie


Janine 2 years ago

Hi Jamie,

This is great! I'm busy with an anatomy and physiology project and wondered if I could have your permission to use some of these images please? I'll make sure to reference appropriately. I'm really struggling to find websites with images that don't have a copyright, and the assignment calls for examples of different staining methods, so if you have some thoughts that would be appreciated.

Cheers,

Janine


jhamann profile image

jhamann 2 years ago from Reno NV Author

Janine-I am sorry to say that I did not properly source my images myself. I would suggest that you go to Histonet. com and ask some Histologists if they know where to obtain images of the different staining techniques. This would probably be a little more approprate for your class. I put this hub together awhile ago and have learned since how to source better. My images were simply taken from google searches. Most of the ones I have are H&E's Histonet will maybe have more options in stains. Keep up a line of communication I am curious to hear how your assignment goes. Jamie


Janine 2 years ago

Thanks Jamie, appreciate you getting back to me.


jhamann profile image

jhamann 2 years ago from Reno NV Author

Your welcome Janine I wish I could have been of more assistance. Try Histonet.org instead of .com, I always get those two mixed up. Jamie


AUPADHYAY profile image

AUPADHYAY 2 years ago from INDIA, UTTAR PRADESH STATE, KANPUR CITY

Lots of information studded in this hub. An interesting and wonderful infohub. Thanks for sharing it jhamann


jhamann profile image

jhamann 2 years ago from Reno NV Author

I hope all is well, thank you for the kind words. Jamie


Shyron E Shenko profile image

Shyron E Shenko 2 years ago

Hi Jamie, this is really interesting. Not that I am going to study this but I seem to come in contact with a lot of this information, i.e. my cousin with coushings and an up hub of my own (no title but the continuation to Jackie's story). And my father died from leukemia, and my hubby has to have Aranesp shots. So this is very interesting.


Tom Schumacher profile image

Tom Schumacher 2 years ago from Huntington Beach, CA

Wow, that was a thorough yet succinct presentation of tissue identification. This hub reminds me of biology class (from many years ago). Is there a specific microscope that you would recommend – both manufacturer and model # - between $600-$1000?


tobusiness profile image

tobusiness 2 years ago from Bedfordshire, U.K

Jamie, excellent information, this could be very useful and not only for students of histology, I could have used this info in my nurse training about a hundred years ago. :) Your hub is clear and concise. Well done, my best to you all.


jhamann profile image

jhamann 2 years ago from Reno NV Author

Thank you Shyron E Shenko, Tom Schumacker, and Jo for enjoying my hub. Let me get back to you Tom about the microscopes. Jamie

    Sign in or sign up and post using a HubPages Network account.

    0 of 8192 characters used
    Post Comment

    No HTML is allowed in comments, but URLs will be hyperlinked. Comments are not for promoting your articles or other sites.


    Click to Rate This Article
    working