The Enzyme Catalase Activity at Different Conditions Lab Report

Enzyme Catalase
Enzyme Catalase

This is a lab report I wrote for Biology 1 (Molecular and Cells) class at CCBC Essex in Spring 2009. This report is my work, and cannot be used without my permission. If you have any question(s) about this report or find any errors, please don't hesitate to leave me a comment.

** Anyone can use this report as a reference of his/her lab report as long as credit is given to me. But please remember that this lab report isn't perfect nor the best reference for the particular experiment.


Living organisms produce enzymes to speed up their chemical reactions (Hershey, 2009). The activity of these enzymes can be either fast, slow, or stopped according to the factors influencing them. This experiment tested the effect of different surface areas, temperatures, and pHs on the reaction rate of the enzyme Catalase, as well as the reusability of the enzyme.

Following are the hypothesis:

  1. As the surface area gets larger, the reaction rate will increase.
  2. The reaction rate will be the fastest when the temperature is at 37°C, and very high temperature (boiling) will denature the enzymes and therefore no reaction will occur.
  3. The optimum pH will be 7, and very high pH (pH 10) will denature the enzymes.
  4. The enzymes are reusable as long as they are not denatured.

Materials and Methods

 Materials, experimental setup, and procedures can be found on pages 73 to 78 in the Biology 110 Lab Manual.


Part 1 - Decomposition of Hydrogen Peroxide with the Enzyme Catalase

The enzyme Catalases in two banana wedges with different surface areas were used to test the effect of the surface area on the enzyme activity. One banana wedge was mashed to increase the surface area. O2 gas was collected using the experimental setup described in the materials and methods section. Results were recorded on Table 1 and graphed on Graph 1.

Part 2 - Effect of Temperature on Enzyme Function

O2 gas was collected using the experimental setup described in the materials and methods section, the enzyme Catalase in chicken liver, and different temperatures. Different temperatures used were 0°C, room temperature, 37°C, and boiled. The results were recorded on Table 2 and graphed on Graph 2.

Part 3 - Is Catalase Reusable?

The reusability of the enzyme Catalase was tested by pouring unused H2O2 over the chicken liver used for previous part. The reusability of H2O2 was tested by putting a fresh cut chicken liver in the liquid left from the room temperature test tube from Part 2. The result was negative, no reaction occurred.

Part 4 - Reaction Rate of Iced Liver Returned to Room Temperature

The data was collected using the iced liver returned to room temperature. The results were recorded on Table 3.

Part 5 - Effect of pH on Enzyme Activity

The effect of pH on enzyme activity was tested using different pHs (3, 7, and 10). The results were recorded on Table 4 and graphed on Graph 3.

Discussion and Conclusion

Part 1 – The experiment supported the hypothesis: As the surface area gets larger, the reaction rate becomes faster. It is so because as the surface area gets larger, bigger area gets in contact with the substrate and react to it, therefore speeds up the reaction. An analogy would be there is a long line at the bank, and 5 tellers handles the business faster than 1 teller. The Graph 1 displays a big difference in the reaction rate between mashed banana and whole banana. The mashed banana, the banana with more surface area, has faster reaction rate than the whole banana, the banana with less surface area.

Part 2 – The experiment refuted the hypothesis of 37oC being the optimal temperature for the enzyme activity (having the fastest reaction rate). This was due to the uncontrolled lab circumstances. Many people were using the incubator and the temperature wasn’t kept stable at 37oC.

Part 3 – The experiment supported the hypothesis: Catalase was reusable and the hydrogen peroxide wasn’t reusable. Results agree with the established biological facts: reacting substances change, therefore aren’t usable, and enzymes don't change, therefore enzymes are reusable (Hershey, 2009).

Part 4 – The experiment supported the hypothesis: Reaction rate of iced liver returned to room temperature is faster than iced liver. This was so because as long as the enzymes don't get denatured, the enzymes react faster as it gets near the optimum temperature (Brooker, 2008).

Part 5 – The experiment supported the hypothesis: Reaction rate is fastest at pH 7. This was true because the optimum pH for the enzyme Catalase is 7. PH 3 wasn’t close to the optimum pH so the reaction rate was slow, and pH10 was too high and it denatured the enzymes.

Literatures Cited

Brooker, Robert J, et al. Biology. New York: McGraw-Hill, 2008.

Hershey, James, et al. Biology 110 Laboratory Textbook. New York: Pearson, 2009.

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Comments 70 comments

Haunty profile image

Haunty 7 years ago from Hungary

A fantastic report. No questions. It's all clear cut. lol

hyunchang profile image

hyunchang 7 years ago Author

Thank you! :)

lumberjack profile image

lumberjack 7 years ago

I'm inspired to upload a lab report!

jinki 7 years ago


Flinki 7 years ago

The intro is quite short...maybe go into enzyme theory?? Talk about active sites, globular proteins, induced fit and lock and key hypotheses n shiz.

But nice work!!

naz shafeeq 7 years ago

thanks for this, you've been a big help..

some awesome dude 7 years ago

Can i use the surface area part as an indirect quote in my lab report?

hyunchang profile image

hyunchang 7 years ago Author

Yes, as long as you indicate the source. :)

some awesome dude 7 years ago

absolutely! thanks man!

hyunchang profile image

hyunchang 7 years ago Author

No problem :)

7 years ago


Diara 7 years ago

You could have said WHY the surface area affects the rate of reaction

hyunchang profile image

hyunchang 7 years ago Author

Thank you for the suggestion Diara. I will add that to the report.

Ashley 7 years ago


I'm doing a similar lab report for my senior year (high school) biology lab and this is exactly what I needed. Thank you for uploading this lab report.

hyunchang profile image

hyunchang 7 years ago Author


I'm very glad my hub helps!

Anna 7 years ago

can I compare my results to yours in my lab report if cite th source?

hyunchang profile image

hyunchang 7 years ago Author

Yes you can :)

chasity 7 years ago

this info is really helpful! it breaks down this lab a lot easier! thanks

chasity 7 years ago

this info is really helpful! it breaks down this lab a lot easier! thanks

hyunchang profile image

hyunchang 7 years ago Author

Thanks! Comments like yours make me want to post more lab reports. :)

vadaa 7 years ago

nice analogy..

hyunchang profile image

hyunchang 7 years ago Author

Thanks! :)

lucy :)  6 years ago

thankkkkyou buddie yu jst saved my life :D

Yasmin 6 years ago

hmmm thank you wouldn't be enough to say..

there are only few great people left in this world

and sure you are one of them :)

thanks that was helpful

krishna 6 years ago

not tooo complete...

some of the information are still not included

... 6 years ago

where is graph 1?

Ellem 6 years ago

Thank You! You helped me a lot to understand this topic! =)

nxzkd 6 years ago


gie 6 years ago

tq,,, it helps me a lot,,,,

noobcaek 6 years ago

thank! your information was set out so clearly. it's so much better that all those sites that rattle on about nothing. >_

kyowon331 6 years ago

hey thank you for your work but this is my first time where do i find the graph

hyunchang profile image

hyunchang 6 years ago Author

Sorry, I only published the text part of the report :(

yi88oou 6 years ago

you r an idiot

hyunchang profile image

hyunchang 6 years ago Author

I am sorry that you wanted to say that after reading my hub. :( If you tell me why I am an idiot, maybe I can stop being one! :)

jomac 6 years ago

Hi, Thanks for this ,it was so helpful. As were some of the suggestions. Well, apart from the tool who called u an idiot!

tohru 5 years ago

may i ask?? what is the purpose of performing catalase activity?? and for the step "is catalase reusable??

passingby 5 years ago

Wanted to say that I liked how you responded to the "idiot" comment. Nice example.

fluffy diva from ja 5 years ago

omg i still dnt get it>>>>>>>>>> jah knw bio haaad

jamaica land we love..... jamaica nuff problem lol 5 years ago

mi need sum baby waah help mi!!!!!!!!!!! i mean i am in great need of help biology is killing me

Sam 5 years ago

Thanks- I really needed this for a Bio internal

Clozzle 5 years ago

Intro is very short. But thanks

Nimly 5 years ago

Where table 1?

J.G. 5 years ago

Can I have permission to use you as a reference for my lab report? I will cite you, of course!

Farrahhh 5 years ago

wow, AWESOME!!!!!! This is great for my AP Bio class right now!!!! WOOT WOOT! :DDDDDD

Farrahhh 5 years ago

Wait, I can find the graphs!! aaaahhh!!! where are they?

Beverly 5 years ago

Can i please use the surface area part to support that my hypothesis was proven?

aeisha 5 years ago

why is the H2O2 tested its not the enzymes its the subtrate which doesn't need to be reusable.

emma 5 years ago

hey can i cite this experiment to compare with my biology lab in order to prove my results ?

Rhiannon 5 years ago

May I use a quote from your part 2 conclusion to support the optimal temperature in my enzyme lab for IB bio? I will obviously cite you as a resource in MLA format at the end of my report!

KJ 5 years ago

Hello...may i please use this site as a referance to were i got some information from as it helped clear up some thing and helped me beter understand my data?

psd 5 years ago

thanks man...u r a life saver..i got so much ideas from this.

yonkinjum 5 years ago

thanks for letting us see your tables and graphs

paramjeet 5 years ago

we were trying to record catalase activity in broad bean at 4 C but not getting any results does that mean that we should inc the temp to 37 c

jacob 4 years ago

i need to write an introduction for a grade 12 bio lab can i cite you and this lab as a source with your permission??

would be a great help

LOL jacob 4 years ago

bro r u kidding me lol

2253 4 years ago

not helpful

ana 4 years ago

may i use it as a reference to write my report?

hyunchang profile image

hyunchang 4 years ago Author

Yes, you may. But don't blame me if it's not accurate :)

jhgdfsjf 4 years ago


kelecia 6043 bebe 4 years ago

who invented school?

effe 4 years ago

Thank you

selina 4 years ago

would you mind if I used your Lab as a guide/source for mine because we're doing a similar project. I will most definitely cite this! Thanks for uploading. P.s. when is the date of publication? I couldn't seem to find it!

hyunchang profile image

hyunchang 4 years ago Author


Yes, you can use this hub as you desire. This report was never published, but was written in Spring of 2009.

gues 4 years ago

you spelt hypotheses wrong

writing a lab report 4 years ago

Thanks for sharing this is good info :

Lulu 4 years ago

I have a suggestion, you should write, "Here are the hypotheses", as you mentioned more than one hypothesis, and the word 'are' was used in the sentence, suggesting more than one. :)

4 years ago

Thanks, I needed something to compare my data to

12345 4 years ago

Hello sir. May I use your report as a reference for my lab?

Comandolf 3 years ago

Hello. I am gathering secondary data to use in my own report, may i use your results?

lucy 2 years ago

thank you for sharing this. i just wanted to kindly ask what grade did you get after? thank you

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