How To Make Your Own Tempeh Spore Starter For Making Homemade Tempeh

What is Tempeh

Tempeh is made from fermented soybeans. It originated from Indonesia where soy is a staple source of protein for the people and where consumption of meat is considered a luxury. It is a popular vegan food as a substitute for meat, and is gluten-free.

The production of tempeh involves soaking the soybeans for several hours and cooking them before letting them ferment. Fermentation of tempeh is complete within 48 hours when the soybeans are bind together by layers of white mycelium from a species of white fungus called Rhizopus Oligosporus. The solid mass of tempeh can then be cut into slices or chunks for cooking.

Homemade tempeh.
Homemade tempeh. | Source

Finding the Tempeh Starter

If you do not live in Indonesia, finding the tempeh starter can be quite difficult. Tempeh starter is a fermentation starter for fermenting the soybeans and binding them into a cake. The fermentation culture contains the spores of Rhizopus Oligosporus. Starters can be ordered online, at a rather costly price though. If you have never made tempeh before and just want to experiment with making tempeh, you won't want to buy a packet of starter good enough to make 10kg of tempeh. And, you don't even know if you are going to end up with edible tempeh or an epic fail!

Basically, the tempeh starter contains spores of Rhizopus Oligosporus and some rice flour. The tempeh starter is very easy to make. It costs almost next to nothing to make, other than paying for a packet of commercially made tempeh, plus your time and effort. So, you will need to buy a small packet of tempeh to make more tempeh. It is similar to making homemade yogurt - if you want to make yogurt, you need to buy a small tub of yogurt with live culture as a starter.

Note: Some commercially produced tempehs might have been subjected to heat to kill off the fungus and prevent them from sporulating. Look for a different supplier if the previous batch of tempeh does not produce any black spores after incubating.

You can find packets of tempeh in the freezer section in most Asian stores and some supermarkets. I would give the supermarkets a miss because they tend to stock marinated tempeh. We need plain tempeh to make the starter. A packet of store bought tempeh should have traces of spores that are invisible to the naked eye. A milky white tempeh will have a lower spore count as compared to one that has tiny black dots on it. So, if you are after some spores, try to choose a tempeh that looks a little 'dirty' or with traces of black dots. (Any other colours on the tempeh - AVOID! They are probably not fresh.) If you can't find tempeh with black spores, you can make the white tempeh 'mature' and sporulate (grow spores).

Setting Up The Incubator

When you are ready to make the tempeh starter, you will need to improvise an incubator for producing the tempeh starter and for making batches of tempeh. Your homemade incubator must be able to maintain a temperature of 32°C. Test the incubator to see if it can provide a stable temperature for making tempeh later.

How to make an incubator? If you happen to have an old bar fridge or a big insulated container waiting to be discarded, don't throw it out just yet. It is perfect for incubating tempeh because the thick insulated walls minimises heat loss (especially in winter) and it does not require more than 10W to operate. My homemade incubator has a small 10W heating mat (for pets), a hot water bottle and an aquarium thermometer. The heating mat rests on a rack in the centre of the bar fridge, and is covered with some tea towels and another metal rack over it. This prevents direct contact of the tempeh with the heating mat which may cause overheating and uneven heat distribution.

The hot water bottle is placed at the bottom of the fridge and the thermometer at the top of the fridge. Heat from the hot water bottle at the bottom will rise and get distributed all over the fridge, and the temperature is recorded by the thermometer near the freezer at the top of the fridge.

Incubator for making tempeh

Old bar fridge with heating mat and thermometer.
Old bar fridge with heating mat and thermometer. | Source
Layers of cloth and a metal baking rack to prevent the tempeh from direct contact with the heating mat.
Layers of cloth and a metal baking rack to prevent the tempeh from direct contact with the heating mat. | Source
A small chunk of tempeh from commercial tempeh.
A small chunk of tempeh from commercial tempeh. | Source

Making the Tempeh Starter

Cut out a chunk of tempeh from your store bought tempeh and eat the rest of the tempeh. Hopefully this will be the last time you have to eat commercially manufactured tempeh! The small chunk will become the starter for all your homemade tempeh later.

Place the chunk of tempeh in a small bowl and cover with cling wrap. Pierce holes all over the cling wrap with a toothpick and place the bowl of tempeh in the incubator for at least two days.

The piece of tempeh for making the starter is in the incubator. A hot water bottle is placed on the lower rack.
The piece of tempeh for making the starter is in the incubator. A hot water bottle is placed on the lower rack. | Source
The temperature dropped 2 degrees when I opened the door of the fridge. It is now around 30 degrees Celsius. Try not to open the door too often if the room temperature is only 15 degrees like in my house!
The temperature dropped 2 degrees when I opened the door of the fridge. It is now around 30 degrees Celsius. Try not to open the door too often if the room temperature is only 15 degrees like in my house! | Source
White fluff starts to appear on the piece of tempeh after 12 hours in the incubator. That means the fungus is alive and growing!
White fluff starts to appear on the piece of tempeh after 12 hours in the incubator. That means the fungus is alive and growing! | Source
Black and grey spores starting to appear. They are ready to be harvested for making the tempeh starter.
Black and grey spores starting to appear. They are ready to be harvested for making the tempeh starter. | Source

Check the temperature every 5 hours or so to be sure it does not go below 28°C or above 35°C. After 24 hours, you should be able to see some changes on the piece of tempeh, it will either have fluffy white mycelia growing on it or it will have started to turn grey. If it is white and fluffy, the starter is not ready yet. (If it turns slimy and smells foul, it is no good - should be discarded immediately. Start with another fresh chunk of tempeh, that is, if you haven't already eaten the remaining tempeh!)

Leave it in the incubator until traces of black appear. Black means the culture has started to sporulate producing the black spores we are after.

When there is enough grey or black stuff on the piece of tempeh, remove it from the incubator and cut it into thin slices or very small chunks. Leave the chunks to air dry for a couple of days. Blend the dry chunks with double amount of rice flour until they are in powder form. This will be your tempeh starter and it is enough to make at least ten batches of tempeh. The homemade tempeh starter needs to be stored in an airtight container in the fridge. It can stay in the fridge for about a week. For longer storage, put it in the freezer.

Black or white? Both jars have enough spores to make several kilos of homemade tempeh.
Black or white? Both jars have enough spores to make several kilos of homemade tempeh. | Source

The starter on the left was made from a fresh piece of tempeh without visible black spores. Fermentation of tempeh with this starter tends to take longer as compared to fermenting with the black spores starter on the right. In conclusion, if there are black dots all over the tempeh, the spore count is definitely higher.

Ingredients for Making Tempeh

  • 1 cup soybeans, soaked and cooked
  • 1-2 tablespoons vinegar
  • half to one teaspoon homemade tempeh starter
Soybeans - the main ingredient of traditional tempeh.
Soybeans - the main ingredient of traditional tempeh. | Source

Approx. Fermentation Time for Tempeh

  • Prep time: 36 hours 50 min
  • Cook time: 45 min
  • Ready in: 37 hours 35 min
  • Yields: 1 slab of tempeh, 4 servings.

Here's the Exciting Part - You Can Now Make Your Own Homemade Tempeh

At last, you can start making some tempehs. Now that you have already made your own tempeh starter, making your own homemade tempehs will be a breeze, believe me!

Measure one cup of soybeans and soak them for at least 8 hours or overnight. Pour away the water used for soaking and top up with clean water. Boil the soybeans for at least 30 minutes or until they are soft. Another way for softening the soybeans is to boil them for 10 minutes and then transfer them to the thermal cooker to continue cooking for another hour or so.

Drain the water and dehull the soybeans. I find that leaving some hulls with the beans does not affect the taste or quality of the tempeh. Next, add 1 tablespoon of vinegar to the beans. Place the pot of beans over the stove and stir. This process helps to remove the excess water quicker. The beans should not be soaking wet or too dry for making into tempeh.

Set aside the soybeans to cool to room temperature. Add in half to one teaspoon of the homemade tempeh starter and mix well. Transfer the beans to a glass bowl and cover with cling wrap. Prick holes 1-2cm apart on the cling wrap with a toothpick. The bowl of beans is ready for incubation.

Put the bowl in the incubator. Check the temperature every 5-6 hours. Some white stuff should be visible after 12 hours if the temperature is correct. Maintaining the temperature at around 32°C is very important, otherwise the tempeh will not turn out well.

Small clumps of fluffy white mycelium is growing after at least 12 hours.
Small clumps of fluffy white mycelium is growing after at least 12 hours. | Source
More white fluff after another half a day.
More white fluff after another half a day. | Source

A white fluffy layer of mycelium will cover the beans eventually, around 30-36 hours. At this stage, black spores might start to appear in some areas. If not, leave the tempeh a little longer in the incubator.

The tempeh should not smell foul. If it does, discard it because it is not edible anymore. The fermentation normally does not take more than two days, and the mycelium should be distributed all over the beans.

After 30-36 hours, the tempeh is ready when the soybeans are covered with white mycelium.
After 30-36 hours, the tempeh is ready when the soybeans are covered with white mycelium. | Source

When the soy beans are all covered with white mycelium, remove the tempeh from the incubator. The tempeh can be used for cooking right away - freshly made tempeh is irresistible!

Homemade milky white tempeh is ready. The mycelium is evenly distributed and the bottom of the tempeh should be all white as well.
Homemade milky white tempeh is ready. The mycelium is evenly distributed and the bottom of the tempeh should be all white as well. | Source

Please rate this article on making tempeh starter and fermenting tempeh at home

5 stars from 1 rating of Homemade Tempeh from Scratch

What do you know about tempeh?

See results without voting

Tempeh can also be made with other legumes like chickpeas, adzuki beans, black beans, barley and mung beans.The beans can be placed in ziplock bags for fermenting. Remember to pierce holes in the bags before putting them in the incubator. The bags of tempeh can be stored in the freezer for many months. You'll never run out of tempeh again!

Different variety of tempeh in ziplock bags for storing in the freezer.
Different variety of tempeh in ziplock bags for storing in the freezer. | Source
Red bean and barley tempeh, great flavour - one of my favourites!
Red bean and barley tempeh, great flavour - one of my favourites! | Source
Black bean tempeh - took about 48 hours to ferment with a weaker tempeh starter.
Black bean tempeh - took about 48 hours to ferment with a weaker tempeh starter. | Source
Soy tempeh simmering in tomato and chilli sauce.
Soy tempeh simmering in tomato and chilli sauce. | Source

How To Cook Tempeh

  1. Slice and pan fry each side for a few minutes until a little brown.
  2. Add pan-fried tempeh to curry dishes and stews, use them in soups, simmer tempeh in tomato sauce, sweet and sour sauce or dip them in chilli sauce.
  3. Slices of tempeh can also be marinated and then deep fried until crispy. Makes a good snack.
  4. A totally versatile ingredient for cooking!
Pan fried red bean tempeh.
Pan fried red bean tempeh. | Source
Coconut rice served with tempeh and other ingredients.
Coconut rice served with tempeh and other ingredients. | Source

Tempeh

Nutrition Facts
Serving size: 100g
Calories 193
Calories from Fat99
% Daily Value *
Fat 11 g17%
Saturated fat 2 g10%
Unsaturated fat 4 g
Carbohydrates 9 g3%
Sugar 9 g
Protein 19 g38%
Cholesterol 9 mg3%
Sodium 9 mg
* The Percent Daily Values are based on a 2,000 calorie diet, so your values may change depending on your calorie needs. The values here may not be 100% accurate because the recipes have not been professionally evaluated nor have they been evaluated by the U.S. FDA.

© 2015 lady rain

More by this Author


4 comments

peachpurple profile image

peachpurple 14 months ago from Home Sweet Home

oh now i know that tempeh is made from soy beans and vinegar. Taste delicious with chillies


Carb Diva profile image

Carb Diva 14 months ago

Wow, this is a very impressive hub on tempeh. I had no idea that you could make your own. My younger daughter is vegetarian--we enjoy the taste of tempeh but it is quite expensive. I'm not sure I am brave enough to attempt this. Luckily, there is an Asian market not very far from our town and we can obtain good-quality tempeh whenever we want it. Thank you also for the recipes. Have a wonderful day.


lady rain profile image

lady rain 14 months ago from Australia Author

peachpurple, tempeh is originally made from soy but you can use chickpeas, mung beans, barley and other legumes if you make your own tempehs.


lady rain profile image

lady rain 14 months ago from Australia Author

Carb Diva, I'm glad you like this hub on tempeh. I hope you'll be able to make your own tempeh one day. Thank you for the lovely feedback.

    Sign in or sign up and post using a HubPages Network account.

    0 of 8192 characters used
    Post Comment

    No HTML is allowed in comments, but URLs will be hyperlinked. Comments are not for promoting your articles or other sites.


    Click to Rate This Article
    working