Alternative names:

GAMMA-A-GLOBULIN antibody

IgA Heavy Chain antibody

Anti-Mouse Antibody (HAMA); human immunoglobulins specific for mouse immunoglobulins.

The human IgA is a modified protein in the form of glycosylation of 160 kilo Dalton. The antibody is made in the form of a monomer or a J chain in IgA dimer. It is usually produced in the serum, saliva, plasma cells in the bone marrow, GI tract, mammary gland and bronchial fluids.

Applications:

Immunohistochemistry, Don blots, western blots. ELISA, flow cytometric analysis, gel shifts (for super shifts), radioimmunoassay, immunodiffusion.

Secondary antibodies for the above applications are produced against a range of specific mouse immunoglobulin classes such as IgG, IgM or IgA that is able to recognise various fragments of the primary antibodies or whole molecules. The anti-mouse antibody can be conjugated with most commonly with horseradish peroxidase or even with alkaline phosphatase, fluorescein, odamine or biotin. The anti-mouse antibody can be raised in several hosts either in polycolonal usually in the form of whole molecules or fragment monoclonal forms (F(ab')2).