ArtsAutosBooksBusinessEducationEntertainmentFamilyFashionFoodGamesGenderHealthHolidaysHomeHubPagesPersonal FinancePetsPoliticsReligionSportsTechnologyTravel
  • »
  • Education and Science»
  • Elementary, Middle School & High School

Prepared Media Plates - Sterilization Methods

Updated on March 14, 2015

Sterile Prepared Media Plate

Sterile media plates: Modified m TEC, m-FC, and MEI for Enterococci
Sterile media plates: Modified m TEC, m-FC, and MEI for Enterococci | Source

Sterilization: The Absence of All Living Things

Sometimes folks get the words "sterile" confused with disinfected, clean, or even purified.

The truth is: those other terms are not the same as the term, Sterile.

Sterility means, "The absence of all living things."

How many times when you were removing a sliver, and someone (probably your mom), put a needle over an open flame for a few seconds to "sterilize" the needle.

Well, an open flame does go a long ways towards removing pathogenic, (disease causing) bacteria and viruses - so we'll give your mom credit for that one! An open flame treatment does not, in fact create a sterile device, but a disinfected one - which is great for removing slivers!

Hand sanitizers reduce the normal flora (bacteria) on your hands, but they do not eradicate all bacteria from your skin. You would eventually get quite sick if that happened from an imbalance in skin pH among other problems.

When we refer to a sterile plate in the laboratory, even technicians have no way of telling whether or not for sure the prepared media plate or petri dish they are using is truly sterile. That's why we run a sterile control with each batch of bacteria we cultivate to give us a reasonable assurance that the conditions we were working with - plates, media, rinse water, funnels, etc., were all sterilized properly before filtration and analysis.

So, to review...we can "clean" our dog or our car...

We can "sanitize" our hands or face...

We can "Pasteurize" our milk...

But we need to "Sterilize" our prepared media plates before use!

OK - that should help!

Fruits and Veggies can be sterilized with gamma radiation
Fruits and Veggies can be sterilized with gamma radiation

Zapped!!! with GAMMA Rays!

Zapped!!! with  GAMMA Rays!
Zapped!!! with GAMMA Rays!

Typical Filter Manifold Set Up

Filter Manifold with Polysulfone Filter Funnels
Filter Manifold with Polysulfone Filter Funnels

Prepared Media Plates and Colony Counting

Membrane Filtration Sterilization Tips

Of course you're going to sterilize your filter funnels prior to sample filtration. But, if they are sterilized via autoclaving, MAKE SURE the funnels have cooled sufficiently before introducing your sample. Not that big of a deal if you are filtering a full 100 mls., but if you are doing serial dilutions, and some of your aliquots are in the 2-5 milliliter range, you ring the risk of B-B-Qing the bacteria in your sample from the very hot walls of the filter funnels.

Don't do that! Simply rinse the funnel walls first with cool, sterile buffered dilution water. There - problem solved! Maybe that's why you didn't get any growth on that small sample last week!

Prepared Media Plates and Positive Controls

Fruits under Gamma Radiation

    0 of 8192 characters used
    Post Comment

    No comments yet.

    working

    This website uses cookies

    As a user in the EEA, your approval is needed on a few things. To provide a better website experience, hubpages.com uses cookies (and other similar technologies) and may collect, process, and share personal data. Please choose which areas of our service you consent to our doing so.

    For more information on managing or withdrawing consents and how we handle data, visit our Privacy Policy at: "https://hubpages.com/privacy-policy#gdpr"

    Show Details
    Necessary
    HubPages Device IDThis is used to identify particular browsers or devices when the access the service, and is used for security reasons.
    LoginThis is necessary to sign in to the HubPages Service.
    Google RecaptchaThis is used to prevent bots and spam. (Privacy Policy)
    AkismetThis is used to detect comment spam. (Privacy Policy)
    HubPages Google AnalyticsThis is used to provide data on traffic to our website, all personally identifyable data is anonymized. (Privacy Policy)
    HubPages Traffic PixelThis is used to collect data on traffic to articles and other pages on our site. Unless you are signed in to a HubPages account, all personally identifiable information is anonymized.
    Amazon Web ServicesThis is used to collect data on traffic to articles and other pages on our site. Unless you are signed in to a HubPages account, all personally identifiable information is anonymized. (Privacy Policy)
    CloudflareThis is used to quickly and efficiently deliver files such as javascript, cascading style sheets, images, and videos. (Privacy Policy)
    Google Hosted LibrariesJavascript software libraries such as jQuery are loaded at endpoints on the googleapis.com or gstatic.com domains, for performance and efficiency reasons. (Privacy Policy)
    Facebook LoginYou can use this to streamline signing up for, or signing in to your Hubpages account. No data is shared with Facebook unless you engage with this feature. (Privacy Policy)
    PaypalThis is used for a registered author who enrolls in the HubPages Earnings program and requests to be paid via PayPal. No data is shared with Paypal unless you engage with this feature. (Privacy Policy)
    Google AdSense Host APIThis service allows you to sign up for or associate a Google AdSense account with HubPages, so that you can earn money from ads on your articles. No data is shared unless you engage with this feature. (Privacy Policy)
    Features
    Google Custom SearchThis is feature allows you to search the site. (Privacy Policy)
    Google MapsSome articles have Google Maps embedded in them. (Privacy Policy)
    Google ChartsThis is used to display charts and graphs on articles and the author center. (Privacy Policy)
    Google YouTubeSome articles have YouTube videos embedded in them. (Privacy Policy)
    VimeoSome articles have Vimeo videos embedded in them. (Privacy Policy)
    MavenThis supports the Maven widget and search functionality. (Privacy Policy)
    Marketing
    Google AdSenseThis is an ad network. (Privacy Policy)
    Google DoubleClickGoogle provides ad serving technology and runs an ad network. (Privacy Policy)
    Index ExchangeThis is an ad network. (Privacy Policy)
    SovrnThis is an ad network. (Privacy Policy)
    Facebook AdsThis is an ad network. (Privacy Policy)
    Amazon Unified Ad MarketplaceThis is an ad network. (Privacy Policy)
    AppNexusThis is an ad network. (Privacy Policy)
    OpenxThis is an ad network. (Privacy Policy)
    Rubicon ProjectThis is an ad network. (Privacy Policy)
    TripleLiftThis is an ad network. (Privacy Policy)
    Say MediaWe partner with Say Media to deliver ad campaigns on our sites. (Privacy Policy)
    Remarketing PixelsWe may use remarketing pixels from advertising networks such as Google AdWords, Bing Ads, and Facebook in order to advertise the HubPages Service to people that have visited our sites.
    Conversion Tracking PixelsWe may use conversion tracking pixels from advertising networks such as Google AdWords, Bing Ads, and Facebook in order to identify when an advertisements has successfully resulted in the desired action, such as signing up for the HubPages Service or publishing an article on the HubPages Service.
    Statistics
    Author Google AnalyticsThis is used to provide traffic data and reports to the authors of articles on the HubPages Service. (Privacy Policy)
    ComscoreComScore is a media measurement and analytics company providing marketing data and analytics to enterprises, media and advertising agencies, and publishers. Non-consent will result in ComScore only processing obfuscated personal data. (Privacy Policy)
    Amazon Tracking PixelSome articles display amazon products as part of the Amazon Affiliate program, this pixel provides traffic statistics for those products (Privacy Policy)