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Armadillidium vulgare and measuring its taxic response to light and humidity
Armadillidium vulgare and it's taxic responses
The purpose of this experiment was to determine whether isopods, in this case Armadillidium vulgare, would display a significant response to humidity and light. Based on the location and habitat of these crustaceans we hypothesized that they would move away from the light and migrate towards moisture. This is... known as positive hydrotaxis and negative phototaxis. In our experiment we had six groups with ten isopods each. The apparatus used for the experiment was two pairs of fused petri dishes, one modified for studying photo taxis, the other for studying hydro taxis. Each group measured the movements of the isopods over a period of ten minutes each for both modified Petri dishes. The data was averaged and the chi-square statistical formula was used to determine whether the response to light and moisture was significant. The critical value of 3.8 was exceeded both on the hydro taxis and photo taxis portion of the statistical analysis, demonstrating that there was a significant response to these conditions.
Taxis are defined as generated movement in response to a stimulus.
The movement of organisms in response to changes in environment, response to stimuli has always been intriguing to man. In our experiment we will study a significantly smaller organism, isopods, and study their movements or taxis. Taxis are defined as generated movement in response to a stimulus. The stimulus could be light, humidity, temperature or food. In this experiment we will use light and humidity to observe the taxis of these Armadillidium vulgare.
Isopods in Greek means “similar or equal foot” (Fossweb). A.vulgare is commonly known as roly poly, pill bug, sow bug. It’s related to other isopods like crabs, shrimps and lobsters. This isopod lives in dark, damp environments and feeds on decaying matter. A wet environment is important because isopods use gills to breathe. Based on the environment that A. vulgare lives in, my hypothesis was that our isopods would demonstrate positive hydrotaxis and negative phototaxis. A study conducted on isopods by Waloff (1941) indicated that they are sensitive to humidity and light. In her study the isopods demonstrated positive hydrotaxis and negative phototaxis.
Materials and methods
The class was divided into six groups with the groups randomly receiving ten A.vulgare each. The petri dishes modified for phototaxis had one side lid covered with black paint to block out light with a hole in-between to allow the isopods to travel to the unpainted side. The pair of petri dishes modified for hydrotaxis were fused together with a hole in between, however a moistened paper towel was placed on one end with the other side left dry. The paper towel was moistened with ten drops of tap water. When the isopods were introduced to each pair of dishes, care was taken to place lids carefully so that no A.vulgare were crushed.
After the isopods were introduced to the petri dishes one person from each group timed the movement of the crustacean ten times at one minute intervals. This was done to measure both hydrotaxis and phototaxis. The results were posted on the chalk board so that everybody could see the data from each others group. The data from all six groups were broken down into columns for each of the conditions measured, wet/dry, dark/light. The results from all the groups were averaged and entered into the chi-square test statistical formula. The formula was used in order to determine whether there was a significant response from the isopods according to their environmental conditions.
Table 1, 2, and figure 3
Six groups with ten A.vulgare apiece participated in the phototaxis and hydrotaxis experiment. In the phototaxis experiment 42.6 out of 60 A.vulgare demonstrated negative phototaxis. In the hydrotaxis experiment 44.5 out of 60 A.vulgare exhibited positive hydrotaxis. Using the chi-square statistical formula, the critical values for the phototaxis results was 14.02. The critical values for the hydrotaxis results were 10.5. The degrees of freedom were one with the critical value of X² being 3.8. The results in both experiments demonstrated a significant response to the stimulus, thereby rejecting Ho hypothesis. This indicated in our experiment that the isopods favor dark, moist conditions over dry and well lighted.
My hypothesis before the experiment stated that the isopods used in our experiment would demonstrate positive hydrotaxis and negative phototaxis. I had based my presumptions on the environments where A.vulgare would normally be found. They’re normally found under rocks and logs where the conditions are moist and wet. I also knew that isopods use gills to respirate, indicating that they would need to live in a wet environment. The results of the experiment indicated that more isopods migrated towards the wet and dark parts of the petri dishes. The calculated value for each of the experiments was higher than the critical value, indicating that the results were significant. This meant that should reject the null hypothesis, indicating there would be know significant difference, and accept the alternative hypothesis.
A similar experiment conducted by Waloff in 1941 measured the effects of humidity on Porcellio scaber, Dendrocoleum lacteum and Armadillidium vulgare. The experimentors used a rectangular dish with a plate of glass covering the dish. A sulfuric acid of a specific gravity was placed underneath the zinc floor on either side of the dish to raise the humidity inside the container. The experiment demonstrated that as the humidity increased, the isopods gradually stopped moving and stayed where it was the most humid. Waloff also came to the same conclusion while using Armadillidium vulgare and Oniscus asellus. All these isopods lived in similar habitats, around rotting wood, decaying matter in dark, moist conditions. All these isopods demonstrated postitive hydrotaxis nad negative phototaxis.
Our experiment was a lot simpler than the one done by Waloff. However, if more time was available we could have monitored the diet and age of isopods in order to determine whether these factors could have had an impact in the experiment. We could have also varied the distance of the light to the petri dishes in order to expose the isopods to radiant heat.
WALOFF, NADEJDA T1 The Mechanisms of Humidity Reactions of Terrestrial Isopods JF Journal of Experimental Biology JO J Exp Biol YR 1941 FD August 1 VO 18 IS 2 SP 115 OP 135 UL
© 2008 Augustine A. Zavala