Promising treatment methods make the original century pandemic of HIV/AIDS
Researchers at Temple University, Pennsyvania, United States of America, had using editing tools gene CRISPR/Cas9 to complete removal of the HIV-1 genome out virus-infected immune cells of the patient. It's a special achievement can have important implications in the treatment of AIDS and other diseases related to another retrovirus.
CRISPR/Cas9 is often regarded as a tool to remove the disease due to genetics or as a method of gene transfer. New research suggests that CRISPS/Cas9 promises bring good results in removing the virus inserted the genetic codes into host cells. Research results are published in the Scientific Journal Reports today 4/3.
The other common virus, retrovirus inserted copy of their genome into the host cell to reproduce.Drug resistance retrovirus can effectively control the reproduction of the HIV virus after infection, but when patients stop taking the drug, they recur very fast. When to stop treatment, HIV virus continued to predominate, weakens the immune system, from which the onset immunodeficiency syndrome (AIDS).
For many years, the scientists always attempts to remove the virus HIV out of the infected CD4 + T cells, a type of leukemia cells help resistance to infection. Much of the effort to destroy does not bring success. The advent of CRISPR/Cas9 in recent time has inspired researchers develop new approach
Geneticists Kamel Khalili and colleagues from Temple University to extract virus-infected T cells from patients. The team adjusted CRISPR/Cas9 tool dedicated to the DNA of the HIV-1. First, the lead paragraph ARN will find the genetic material of the virus in the whole genome of t. cells after complete identification process, an enzyme from human will remove malicious code from DNA of T cells, DNA repair tool integrated in the cell will reconnect the two ends of the broken DNA.
The method helps to permanently remove the virus DNA segment out of the cell because the protection system is still super small gene in the cell, preventing the infection status when HIV-1 molecules try to penetrate into the cells not yet edited.
The experiment was conducted on T cells is in the disk, but this technique successfully reduced the amount of virus in the cell extract of patients, promises to become effective treatments. However, researchers need more time to develop this. They also eliminate the ability does not meet the expected targets (such as adverse effects of gene editing) and potential toxicity. They proved the cells develop and function normally after removal of HIV-1.
"The findings showed that the effectiveness of the system of our gene editing in the exclusion of HIV from the DNA of CD4 T cells by introducing mutations into the genome of the virus, making it permanently lost the ability to reproduce," Khalili proclaimed. "Moreover, the study points out the system can protect the cells from the infection and this technology is safe for the cells thanks to no toxicity".
Foreign DNA removal technique has many implications for related research, including cancer-causing retrovirus treatment white blood and, as well as the retrovirus are affecting livestock and crops.