Article Review:Transcripts involved in calcium signaling and telencephalic neuronal fate
Chen., H., DeLong, C., Bame, M., Rajapakse, I., Herron, T., McInnis, M., Shea, O.(2014). Transcripts involved in calcium signaling and telencephalic neuronal
In this paper, Chen et al evaluates key changes in gene expression when iPSC which is retrieved from well featured patient mutate into neurons. Accordingly, the authors establish a low variation in iPSC transcriptome while BP neurons slightly differed with the controls in the transcriptional profile. In addition, transcript expressions for ion channels and receptors with membrane bound increased on a significant level for BP-derived neurons when compared with their controls. The authors also noted that the pretreatment of BP neurons using lithium caused a significant alteration to the wave amplitude and calcium transient. The factors responsible for the expression of transcription regarding the specifical of the telencephalic neuronal identity were as well changed. Further, control neurons expressing transcripts conferred fate in dorsal telencephalic while BP neurons expressed the kind of genes which were involved in the segregation of the ventral regions. Cells were found to be involed in the ventral patterning cues as a supplement to the dorsalizing agent or the ventral pathway activator which is a stimulated expression of the dorsal EMX2, and NKX2-1 on both of these groups.
The general observation from this research is that cell based models which in this case can be referred as stem cells does have an essential impact regarding the comprehension of the genesis, hence treatment of bipolar disorder. Accordingly, the study establishes that the cell lines in iPSC offer a critical resource for comparing their effects in treatments with other types of neurodevelopmental conditions.