As a believer in Jesus, I would begin by noting that the Gospel of John was written a couple of hundred years after the death of Jesus, and contains many ideas that we have no idea whether or not they match Jesus' actual teachings.
This one idea, though, does seem sound and corresponds with teachings of Jesus in the earlier Canonical Gospels, Matthew, Mark, and Luke.
I believe that, within the New Testament, the best guides to worshipping God in Spirit and in Truth are the Lord's Prayer and the Beatitudes from the Sermon on the Mount. The Beatitudes are best an in-depth study, so I'll look at the Lord's Prayer.
Jesus' disciples asked, "Teach us to Pray," and Jesus replied by teaching the Lord's Prayer, which starts, "Our Father Who art in Heaven." This is stating a truth, and stating that God is in the spiritual realm, Heaven. "Hallowed be Thy Name. Thy Kingdom come, Thy Will be done, on Earth as it is in Heaven." This is asking that God's spiritual truth become true here on Earth. We then ask God for our daily bread, recognizing the truth that he is the sustenance of all. We also ask that, when temptation comes, he lead us through it and deliver us from evil, fulfilling His spiritual purpose.
If we pray this prayer, we've done the first step in worshiping God in Spirit and in Truth. The second step is guided by Jesus' teaching, "Those who hear my words and do as I say build their house on rock." Let us act in harmony with Lord's Prayer, dwelling endlessly in gratitude for life and sustenance, and bearing through temptation, and if we fall again, repenting, so that we may be delivered from evil. Then, indeed, we are worshipping in Spirit and in Truth.